Article
Determination of Helicobacter pylori cagA, vacA genotypes with real-time PCR melting curve analysis.
Journal of physiology, Paris - 1 Jan 2000
Ruzsovics A, Molnar B, Unger Z, Tulassay Z, Pronai L
Abstract excerpt
OBJECTIVES: genotypes of Helicobacter pylori are the focus of interest because they play a prominent role in mucosal injury. The purpose of this study was to determine cagA and vacA genotypes of H. pylori using real-time polymerase chain reaction (PCR) method with a double strain DNA binding SYBR Green I.dye, and to compare this with those of two immunohistochemical methods. METHODS: forty-three paraffin-embedded...
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