Article
Dual Y-chromosome painting and immunofluorescence staining of archival human liver transplant biopsies.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society - 1 Oct 2001
Dundas S R, Boyle S, Bellamy C O, Hawkins W, Garden O J, Ross J A, Bickmore W
Abstract excerpt
Combining fluorescence in situ hybridization (FISH) and indirect immunofluorescence staining of protein markers provides a highly specific method for identifying chromosomes in phenotypically defined cells and tissues. We developed a technique enabling dual chromosome painting and immunofluorescence staining of archival formalin-fixed, paraffin-embedded material, and used this to phenotype chimeric cells in...
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