Detection of <i>Slackia exigua, Mogibacterium timidum</i> and <i>Eubacterium saphenum</i> from pulpal and periradicular samples using the Polymerase Chain Reaction (PCR) method

Abstract excerpt

AIM: The purpose of this study was to detect Slackia exigua from root canal samples using a sensitive PCR amplification method. Mogibacterium timidum and Eubacterium saphenum were also included because of their culture-difficult properties. METHODOLOGY: The species-specific PCR primers were prepared according to 16S rDNA sequence analysis data, and confirmed to be effective for PCR amplification as...

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