Article
[Prevalence of major virulence genes among various Vibrio cholerae El-TOR strains for evaluating their epidemiological significance].
Molekuliarnaia genetika, mikrobiologiia i virusologiia - 1 Jan 2001
Smirnova N I, Kirillina O A, Cheldyshova N B, Kutyrev V V
Abstract excerpt
Specific oligonucleotide primers were chosen for identifying the fragments of the four major virulence genes of V. cholerae eltor (ctxA, tcpA, toxR, and hap) using the polymerase chain reaction (PCR). In order to estimate the efficiency of complex PCR testing of V. cholerae for evaluation of their epidemiological significance, a collection of 80 V. cholerae eltor strains with known virulence was selected, whose...
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