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Novel Method for Detection, Typing, and Quantification of Human Papillomaviruses in Clinical Samples

1 Sept 2001

Abstract excerpt

We report the development of a novel detection and typing methodology for human papillomaviruses (HPV) based on real-time PCR with the self-probing fluorescent primers known as Scorpions. This technique is quick, simple, specific, sensitive, and capable of estimating viral load per cell. We report the results of over 100 typing reactions performed on cell lines, biopsies, and cervical cytobrush samples which,...

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PMID: 11526152
DOI: 10.1128/jcm.39.9.3204-3212.2001

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