Article
Detection and typing of TT virus DNA genotype by the PCR-RFLP method.
Molecular and cellular probes - 1 Aug 2001
Tanaka T, Kuroda K, Kobayashi M, Sato K
Abstract excerpt
TT virus (TTV) is a novel single-stranded DNA virus that was identified in patients with post-transfusion-hepatitis of non-A-G type in Japan in 1997. We developed a new detection and typing method for TTV DNA strains, by combining polymerase chain reaction (PCR) and restriction fragment length polymorphism (PCR-RFLP) techniques. TTV DNA was amplified by nested-PCR with TTV-specific mixed primers derived from the...
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