New restriction fragment length polymorphism (RFLP) markers for Aspergillus fumigatus.

doi:10.1111/j.1574-695X.2001.tb01580.x

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Article

New restriction fragment length polymorphism (RFLP) markers for Aspergillus fumigatus.

FEMS immunology and medical microbiology - 1 Jul 2001

Semighini C P, Delmas G, Park S, Amstrong D, Perlin D, Goldman G H

Abstract excerpt

In this study, we isolated and tested restriction fragment length polymorphism (RFLP) markers for Aspergillus fumigatus based on PCR products amplified by the random amplified polymorphic DNA (RAPD) primer R108. Four DNA fragments, Afd, Af5, Af4, and Af4A, were amplified. Fragments Afd and Af5 were 85% and 88% identical at the DNA level to part of the Afut1 retrotransposon from A. fumigatus. Fragment Af4A is a...

Topics

Amino Acid Sequence
Aspergillosis
Aspergillus fumigatus
DNA Primers
DNA, Fungal
Genetic Markers
Genotype
Humans
Molecular Sequence Data
Polymorphism, Restriction Fragment Length
Sequence Alignment

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PMID: 11476976
DOI: 10.1111/j.1574-695X.2001.tb01580.x

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