Article
Origin, diversity and evolution of NBS-type disease-resistance gene homologues in coffee trees (Coffea L.).
Molecular genetics and genomics : MGG - 1 Jun 2001
Noir S, Combes M C, Anthony F, Lashermes P
Abstract excerpt
The majority of plant disease-resistance genes (R-genes) isolated so far encode a predicted nucleotide-binding site (NBS) domain. NBS domains related to R-genes show a highly conserved backbone of amino acid motifs, which makes it possible to isolate resistance gene analogues (RGAs) by PCR with degenerate primers. Multiple combinations of primers with low degeneracy, designed from two conserved motifs in the NBS...
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