Article
Molecular characterization of Brazilian infectious bursal disease viruses.
Avian diseases - 1 Jan 2000
Ikuta N, El-Attrache J, Villegas P, García E M, Lunge V R, Fonseca A S, Oliveira C, Marques E K
Abstract excerpt
A reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was used to amplify a VP2 gene fragment (248 bp) from infectious bursal disease virus (IBDV). The procedure allowed the detection of known IBDV strains from the United States, along with field isolates and commercial vaccines produced in Brazil. Amplified VP2 fragments were further characterized by restriction fragment length polymorphism (RFLP)...
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