Article
Mutational analysis of the proteinase function of Potato leafroll virus.
The Journal of general virology - 1 Jun 2001
Sadowy Ewa, Juszczuk Marek, David Chantal, Gronenborn Bruno, Hulanicka M Danuta
Abstract excerpt
cDNA expression vectors of Potato leafroll virus (PLRV) were used to analyse specific mutations in the proteinase and replicase domains of the proteins encoded by ORF1 and ORF2. Agrobacterium-mediated DNA transfer was used to introduce a PLRV RNA expression unit, controlled by the 35S promoter of Cauliflower mosaic virus, into potato leaf cells. Expression of unmodified PLRV cDNA led to the replication of viral...
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