Article
Automated constant denaturant capillary electrophoresis applied for detection of KRAS exon 1 mutations.
BioTechniques - 1 May 2001
Bjørheim J, Ekstrøm P O, Fossberg E, Børresen-Dale A L, Gaudernack G
Abstract excerpt
In this study, we have applied automated constant denaturant capillary electrophoresis (ACDCE) for the detection of KRAS exon 1 mutations. Samples from 191 sporadic colon carcinomas previously analyzed for KRAS mutations with allele-specific PCR (ASPCR), temporal temperature gradient electrophoresis (TTGE), and constant denaturant capillary electrophoresis (CDCE) were analyzed. In ACDCE, an unmodified ABI Prism...
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