Article
A WbpL mutant of Pseudomonas putida DOT-T1E strain, which lacks the O-antigenic side chain of lipopolysaccharides, is tolerant to organic solvent shocks.
Extremophiles : life under extreme conditions - 1 Apr 2001
Junker F, Rodríguez-Herva J J, Duque E, Ramos-González M I, Llamas M, Ramos J L
Abstract excerpt
Lipopolysaccharides (LPS) are major components of the outer membrane of gram-negative bacteria and are considered a defense barrier. To determine if LPS play a role in resistance to solvents in the solvent-tolerant Pseudomonas putida DOT-T1E strain, we have generated mutants unable to synthesize the O-antigen side chain of LPS. The wbpL gene, encoding the enzyme that begins the synthesis of the O-antigen side...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- DNA Primers
- DNA, Recombinant
- Glycosyltransferases
- Microbial Sensitivity Tests
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- O Antigens
- Organic Chemicals
- Phenotype
- Pseudomonas putida