Article
The preparation of periapical lesions for flow cytometry.
International endodontic journal - 1 Mar 2000
Fernando K, Gulabivala K, Barrett A W
Abstract excerpt
AIM: To devise an optimal protocol and to analyse the leucocyte composition of periapical (PA) lesions by flow cytometry. METHODOLOGY: PA lesions were mechanically agitated, with and without proteolysis. This was with either 0.2% collagenase alone, or in combination with 0.02% DNA-ase in serial incubations until all tissue was digested. The efficacy of each method was assessed by counting total cell yield and...
Topics
- Antigens, CD
- Cell Separation
- Cell Survival
- Collagenases
- Deoxyribonucleases
- Flow Cytometry
- Histocytological Preparation Techniques
- Humans
- Leukocytes
- Periapical Periodontitis
- Phenotype