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Article

The preparation of periapical lesions for flow cytometry.

International endodontic journal - 1 Mar 2000

Fernando K, Gulabivala K, Barrett A W

Abstract excerpt

AIM: To devise an optimal protocol and to analyse the leucocyte composition of periapical (PA) lesions by flow cytometry. METHODOLOGY: PA lesions were mechanically agitated, with and without proteolysis. This was with either 0.2% collagenase alone, or in combination with 0.02% DNA-ase in serial incubations until all tissue was digested. The efficacy of each method was assessed by counting total cell yield and...

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Identifiers

PMID: 11307450
DOI: 10.1046/j.1365-2591.2000.00294.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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