Construction of Agrobacterium strains by electroporation of genomic DNA and its utility in analysis of chromosomal virulence mutations.

doi:10.1128/aem.60.11.4192-4194.1994

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Article

Construction of Agrobacterium strains by electroporation of genomic DNA and its utility in analysis of chromosomal virulence mutations.

Applied and environmental microbiology - 1 Nov 1994

Charles T C, Doty S L, Nester E W

Abstract excerpt

We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chr...

Topics

Agrobacterium tumefaciens
DNA, Bacterial
Electroporation
Genome, Bacterial
Mutation
Transformation, Bacterial
Virulence

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