Article
Identification of four structural genes and two putative promoters necessary for utilization of naphthalene, phenanthrene, fluoranthene by Sphingomonas paucimobilis var. EPA505.
Gene - 30 Dec 2000
Story S P, Parker S H, Kline J D, Tzeng T R, Mueller J G, Kline E L
Abstract excerpt
Sphingomonas paucimobilis var. EPA505 utilizes fluoranthene (FLA), naphthalene (NAP), and phenanthrene (PHE) as sole carbon sources for energy and growth. A genetic library of EPA505 was constructed using mini-Tn5 promoter reporter genes encoding for tetracycline resistance (tc(p-)) or luminescence (luxAB(p-)). Out of 2250 Tn5 mutants, ten were deficient in utilization of FLA, NAP, and/or PHE as sole carbon...
Topics
- Bacterial Proteins
- Base Sequence
- Cell Division
- Culture Media
- DNA Transposable Elements
- DNA, Bacterial
- Dioxygenases
- Fluorenes
- Genes, Bacterial
- Hydrocarbons, Aromatic
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutation