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Isolation and characterization of Tn5-induced mutants of Pseudomonas paucimobilis UT26 defective in gamma-hexachlorocyclohexane dehydrochlorinase (LinA).

Bioscience, biotechnology, and biochemistry - 1 May 1993

Nagata Y, Imai R, Sakai A, Fukuda M, Yano K, Takagi M

Abstract excerpt

Pseudomonas paucimobilis UT26 grows on gamma-hexachlorocyclohexane (gamma-HCH) as a sole source of carbon and energy. Tn5 mutation was introduced into UT26, and two kinds of mutants defective in gamma-HCH degradation were phenotypically isolated; one (UT64) completely lacked the activity to degrade gamma-HCH, while the other (UT61) retained a very low level of activity. Tagging and sequencing analysis showed that...

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PMID: 7686793
DOI: 10.1271/bbb.57.703

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