Article
Isolation and characterization of Tn5-induced mutants of Pseudomonas paucimobilis UT26 defective in gamma-hexachlorocyclohexane dehydrochlorinase (LinA).
Bioscience, biotechnology, and biochemistry - 1 May 1993
Nagata Y, Imai R, Sakai A, Fukuda M, Yano K, Takagi M
Abstract excerpt
Pseudomonas paucimobilis UT26 grows on gamma-hexachlorocyclohexane (gamma-HCH) as a sole source of carbon and energy. Tn5 mutation was introduced into UT26, and two kinds of mutants defective in gamma-HCH degradation were phenotypically isolated; one (UT64) completely lacked the activity to degra...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Culture Media
- DNA Transposable Elements
- Gene Expression Regulation, Bacterial
- Genetic Complementation Test
- Hexachlorocyclohexane
- Lyases
- Molecular Sequence Data
- Mutation
- Phenotype
- Pseudomonas
- Restriction Mapping