Article
Use of stopped-flow spectrophotometry to establish midpoint potentials for redox proteins.
Analytical biochemistry - 1 Dec 2000
Sørlie M, Seefeldt L C, Parker V D
Abstract excerpt
Stopped-flow spectrophotometry was examined as a tool to assign midpoint potentials to protein redox half-reactions. The method involves the rapid mixing of protein and electron transfer mediator solutions and the determination of the absorbance of at least one of the reacting species or products at equilibrium. The utility of the method was demonstrated with two different redox proteins (nitrogenase iron protein...
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