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Article

Screening for and characterization of phospholipase A1 hypersecretory mutants of Tetrahymena thermophila.

Applied microbiology and biotechnology - 1 Sept 2000

Hartmann M, Guberman A, Florin-Christensen M, Tiedtke A

Abstract excerpt

We have described a procedure for the isolation of mutants of Tetrahymena thermophila with hypersecretion of phospholipase A1 (PLA1). Using random chemical mutagenesis, uniparental cytogamy, genetic crossing and a new, fast and effective screening procedure, four PLA1-hypersecretory mutants were isolated. The screening procedure is based on the formation of a halo appearing around cylindrical holes in a...

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PMID: 11030577
DOI: 10.1007/s002530000405

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