Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Rapid Detection and Identification of<i>Candida</i>,<i>Aspergillus</i>, and<i>Fusarium</i>Species in Ocular Samples Using Nested PCR

1 Aug 2000

Abstract excerpt

A protocol for the rapid detection of fungal DNA in ocular samples, derived from three species, Candida albicans, Aspergillus fumigatus, and Fusarium solani, has been developed. Two novel panfungal primers complementary to 18S rRNA sequences present in all three species were designed. Panfungal PCR was followed by three nested PCRs utilizing species-specific primers. PCR sensitivity ranged from 50 to 100 fg of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Antifungal resistance and susceptibility
Share to Infectious Diseases and Mycology
Share to Ocular Infections and Treatments

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 10921948
DOI: 10.1128/jcm.38.8.2902-2908.2000

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Rapid Detection and Identification of<i>Candida</i>,<i>Aspergillus</i>, and<i>Fusarium</i>Species in Ocular Samples Using Nested PCRPMID 10921948Read ↗

Application of semi-nested polymerase chain reaction targeting internal transcribed spacer region for rapid detection of panfungal genome directly from ocular specimensSemantic proximity Read ↗

Utility of Molecular Sequence Analysis of the ITS rRNA Region for Identification of<i>Fusarium</i>spp. from Ocular SourcesSemantic proximity Read ↗

PCR identification system for the genus<i>Aspergillus</i>and three major pathogenic species:<i>Aspergillus fumigatus</i>,<i>Aspergillus flavus</i>and<i>Aspergillus niger</i>Semantic proximity Read ↗

<i>Fusarium</i> keratitis in South India: causative agents, their antifungal susceptibilities and a rapid identification method for the <i><scp>F</scp>usarium solani</i> species complexSemantic proximity Read ↗

Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5.8S Ribosomal DNA Typing in Ocular InfectionsSemantic proximity Read ↗

Identification of <i>Aspergillus</i> and Mucorales in formalin-fixed, paraffin-embedded tissue samples: Comparison of specific and broad-range fungal qPCR assaysSemantic proximity Read ↗

Molecular diagnostic platforms for detecting<i>Aspergillus</i>Semantic proximity Read ↗

Spectrum of<i>Fusarium</i>infections in tropical dermatology evidenced by multilocus sequencing typing diagnosticsSemantic proximity Read ↗

Detection of Candida and Aspergillus species DNA using broad-range real-time PCR for fungal endophthalmitisSemantic proximity Read ↗

Development and Clinical Application of a Panfungal PCR Assay To Detect and Identify Fungal DNA in Tissue SpecimensSemantic proximity Read ↗

Panfungal PCR and Multiplex Liquid Hybridization for Detection of Fungi in Tissue SpecimensSemantic proximity Read ↗

Specific Detection of <i>Fusarium</i> Species in Blood and Tissues by a PCR TechniqueSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.