Article
Systematic circular permutation of an entire protein reveals essential folding elements.
Nature structural biology - 1 Jul 2000
Iwakura M, Nakamura T, Yamane C, Maki K
Abstract excerpt
The importance of chain connectivity in determining protein function and stability can be examined by breaking the peptide backbone using a technique such as circular permutation. Cleavage at certain positions results in a complete loss of the ability of the protein to fold. When such cleavage sites occur sequentially in the primary structure, we call the region a 'folding element', a new concept that could...
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