Comparison of pulsed-field gel electrophoresis and coagulase gene restriction profile analysis techniques in the molecular typing of Staphylococcus aureus.

doi:10.1128/JCM.38.6.2186-2190.2000

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Article

Comparison of pulsed-field gel electrophoresis and coagulase gene restriction profile analysis techniques in the molecular typing of Staphylococcus aureus.

Journal of clinical microbiology - 1 Jun 2000

Chiou C S, Wei H L, Yang L C

Abstract excerpt

Pulsed-field gel electrophoresis (PFGE) and coagulase gene restriction profile (CRP) analysis techniques were used to analyze 71 Staphylococcus aureus isolates recovered from nine food-borne disease outbreaks. Twenty-two PFGE profiles and 11 CRPs were identified, with discrimination indices of 0.86 and 0.72, respectively. In addition, the variable regions of the coagulase genes of 39 isolates were sequenced and...

Topics

Bacterial Typing Techniques
Coagulase
Disease Outbreaks
Electrophoresis, Gel, Pulsed-Field
Enterotoxins
Food Handling
Genes, Bacterial
Humans
Latex Fixation Tests
Molecular Epidemiology

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phenotype

Polymorphism, Restriction Fragment Length

Sequence Analysis, DNA

Staphylococcal Food Poisoning

Staphylococcus aureus

Taiwan

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Open in PubMed Open full text

Identifiers

PMID: 10834974
DOI: 10.1128/JCM.38.6.2186-2190.2000
PMCID: PMC86760

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-10834974-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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