Article
Genetic variation in Staphylococcus aureus coagulase genes: potential and limits for use as epidemiological marker.
Journal of clinical microbiology - 1 Oct 1994
Schwarzkopf A, Karch H
Abstract excerpt
To perform coagulase gene typing, the repeated units encoding hypervariable regions of the Staphylococcus aureus coagulase gene were amplified by the PCR technique; this was followed by AluI restriction enzyme digestion and analysis of restriction fragment length polymorphism (RFLP) patterns. In order to assess the discriminatory power of this typing method, 30 epidemiologically unrelated S. aureus strains which...
Topics
- Base Sequence
- Coagulase
- Genes, Bacterial
- Genetic Variation
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Staphylococcus aureus