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Article

Use of gel electrophoresis for the study of enzymatic activities of cold-adapted bacteria.

Journal of microbiological methods - 1 Mar 2000

Berchet V, Boulanger D, Gounot A M

Abstract excerpt

Glutamate dehydrogenase (GDH) and lactate dehydrogenase (LDH) activity of 13 cold-adapted strains, isolated from cold soils and showing GDH and/or LDH activity in spectrophotometric assays, were revealed by the use of electrophoresis on a nondenaturing acrylamide gel (zymogram). Psychrophilic strains were grown at 4 degrees C and 10 degrees C and the psychrotolerant strains at 4 degrees, 20 degrees and 28 degrees...

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PMID: 10739349
DOI: 10.1016/s0167-7012(99)00141-4

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