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Comparison of Virus Isolation and Reverse Transcription Polymerase Chain Reaction Assay for Detection of Bovine Viral Diarrhea Virus in Bulk Milk Tank Samples

1 Mar 2000

Abstract excerpt

The use of a reverse transcriptase polymerase chain reaction (RT-PCR) assay to screen bulk milk tank samples for bovine viral diarrhea virus (BVDV) has proven to be a sensitive and economical means to evaluate the lactating animals in a herd. The assay is capable of detecting the presence of a single persistently infected animal within a group of several hundred cows. Over a 3-year period, 144 samples from 97...

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PMID: 10730955
DOI: 10.1177/104063870001200219

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