Article
Aphid transmission studies using helper component proteins of potato virus Y expressed from a vector derived from potato virus X.
The Journal of general virology - 1 Apr 2000
Sasaya T, Torrance L, Cowan G, Ziegler A
Abstract excerpt
The genes encoding the helper component (HC) proteins of two strains of Potato virus Y (PVY) were cloned and the proteins expressed from a vector derived from Potato virus X (PVX). The expressed HC contained six N-terminal histidine residues to facilitate purification by metal affinity chromatography. Approximately 2-4 microg/g of purified HC was obtained from leaves of Nicotiana benthamiana plants systemically...
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