Article
Transcriptional activation of the cyclooxygenase-2 gene in endotoxin-treated RAW 264.7 macrophages.
The Journal of biological chemistry - 3 Mar 2000
Wadleigh D J, Reddy S T, Kopp E, Ghosh S, Herschman H R
Abstract excerpt
Cyclooxygenase-2 (COX-2), the enzyme primarily responsible for induced prostaglandin synthesis, is an immediate early gene induced by endotoxin in macrophages. We investigated the cis-acting elements of the COX-2 5'-flanking sequence, the transcription factors and signaling pathways responsible for transcriptional activation of the COX-2 gene in endotoxin-treated murine RAW 264.7 macrophages. Luciferase reporter...
Topics
- Adaptor Proteins, Signal Transducing
- Animals
- CCAAT-Enhancer-Binding Proteins
- Cyclic AMP
- Cyclooxygenase 2
- DNA-Binding Proteins
- Drosophila Proteins
- Endotoxins
- Genes, Reporter
- I-kappa B Proteins
- Isoenzymes
- JNK Mitogen-Activated Protein Kinases
- Lipopolysaccharides