Article
Production of recombinant snakehead rhabdovirus: the NV protein is not required for viral replication.
Journal of virology - 1 Mar 2000
Johnson M C, Simon B E, Kim C H, Leong J A
Abstract excerpt
Snakehead rhabdovirus (SHRV) affects warm water fish in Southeast Asia and belongs to the genus Novirhabdovirus by virtue of its nonvirion gene (NV). Because SHRV grows best at temperatures between 28 and 31 degrees C, we were able to use the T7 expression system to produce viable recombinant SHRV from a cloned cDNA copy of the viral genome. Expression of a positive-strand RNA copy of the 11, 550-nucleotide SHRV...
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