Article
Phenylalanine transport in Aspergillus nidulans: demonstration of role of phenylalanine binding proteins.
Indian journal of experimental biology - 1 Feb 1999
Sahay S
Abstract excerpt
Crude shock proteins extracted by two stage osmotic shock were further purified by affinity chromatography to obtain ligand (phenylalanine) specific binding protein (phebip) a component of phenylalanine (phe) transport system from wild type and a phe transport mutant fpaD11 of Aspergillus nidulans. A new eluent 0.1 M Tris-HCl containing 1.5 N NaCl and 0.5 N Na2CO3, pH 8 was used during the investigation. The...
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