Article
Technical advance: single pollen typing combined with laser-mediated manipulation.
The Plant journal : for cell and molecular biology - 1 Nov 1999
Matsunaga S, Schütze K, Donnison I S, Grant S R, Kuroiwa T, Kawano S
Abstract excerpt
We combined single pollen typing with laser-mediated manipulation. After drilling a hole in the wall of a pollen grain from a dioecious plant (Silene latifolia) with a UV-laser microbeam, the single pollen grain was recovered directly in the cap of a PCR tube, using a non-contact method called laser pressure catapulting. The entire genome of the single pollen grain was then amplified with improved...
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