Article
Targeted inactivation of the smallest plastid genome-encoded open reading frame reveals a novel and essential subunit of the cytochrome b(6)f complex.
The EMBO journal - 1 Nov 1999
Hager M, Biehler K, Illerhaus J, Ruf S, Bock R
Abstract excerpt
The smallest conserved open reading frame in the plastid genome, ycf6, potentially specifies a hydrophobic polypeptide of only 29 amino acids. In order to determine the function of this reading frame we have constructed a knockout allele for ycf6. This allele was introduced into the tobacco plastid genome by chloroplast transformation to replace the wild-type ycf6 allele. Homoplasmic Deltaycf6 plants display a...
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