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Article

A structural view of evolutionary divergence.

Proceedings of the National Academy of Sciences of the United States of America - 26 Oct 1999

Spiller B, Gershenson A, Arnold F H, Stevens R C

Abstract excerpt

Two directed evolution experiments on p-nitrobenzyl esterase yielded one enzyme with a 100-fold increased activity in aqueous-organic solvents and another with a 17 degrees C increase in thermostability. Structures of the wild type and its organophilic and thermophilic counterparts are presented at resolutions of 1.5 A, 1.6 A, and 2.0 A, respectively. These structures identify groups of interacting mutations and...

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Identifiers

PMID: 10535917
DOI: 10.1073/pnas.96.22.12305
PMCID: PMC22912

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