Article
A starch-branching enzyme gene in wheat produces alternatively spliced transcripts.
Plant molecular biology - 1 Aug 1999
Båga M, Glaze S, Mallard C S, Chibbar R N
Abstract excerpt
A wheat gene, denoted Sbe1, encoding a type I starch-branching enzyme (SBEI) was isolated from a genomic library and shown to comprise 14 exons distributed over a 5.7 kb DNA region. Analyses of kernel RNA by 5' rapid amplification of cDNA ends (5'-RACE) and reverse transcription-polymerase chain reaction (RT-PCR) demonstrated a considerable sequence variation at the 5' ends of SBEI gene transcripts. DNA sequence...
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