REMI mutagenesis and identification of pathogenic mutants in blast fungus (Magnaporthe grisea).

Abstract excerpt

Blast fungus' mutant, which is very difficult to obtain, is the key substance in the study of the relation between pathogen and plant. Rice-Aichi-ashahi was used as the host and plasmid pBF101 or pCSN43 as foreign DNA fragment, and REMI mutagenesis was applied to tag the pathogenic sequence in rice blast fungus M.grisea. Three mutants (R2H65, R2H69 and R2B1565) related to pathogenicity were recovered from several...

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