Article
Detection of gene deletions in Chinese patients with Duchenne/Becker muscular dystrophy using CDNA probes and the polymerase chain reaction method.
Life sciences - 1 Jan 1999
Yuge L, Hui L, Bingdi X
Abstract excerpt
One hundred thirty-eight patients with Duchenne/Becker muscular dystrophy (DMD/BMD) were screened with complete cDNA probes and the multiplex polymerase chain reaction (mPCR) amplification of 18 pairs of oligonucleotide primers. Eighty-six deletions and 4 duplications were detected, the deletion frequency being 62.3%. Eighty-two deletions were detected with the two sets of primers described by Chamberlain et al....
Topics
- Adolescent
- Adult
- Blotting, Southern
- Child
- Child, Preschool
- China
- DNA, Complementary
- Exons
- Gene Duplication
- Gene Frequency
- Genetic Testing
- Humans
- Introns
- Muscular Dystrophies
- Phenotype
- Polymerase Chain Reaction
- Sequence Deletion