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Quantitation of Hepatitis B Virus Genomic DNA by Real-Time Detection PCR

1 Sept 1999

Abstract excerpt

Quantitation of hepatitis B virus (HBV) DNA in serum is a useful method for the monitoring of HBV replication. We attempted to develop a quantitative assay system for HBV DNA that is more sensitive, accurate, and reproducible than existing systems. We detected HBV DNA by real-time detection PCR (RTD-PCR) based on Taq Man chemistry. The efficacy of this assay was evaluated by quantitatively measuring sequential...

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Identifiers

PMID: 10449472
DOI: 10.1128/jcm.37.9.2899-2903.1999

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