Article
Biomolecular techniques to detect Pneumocystis carinii f. sp. hominis pneumonia in patients with acquired immunodeficiency syndrome.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases - 1 Jan 2000
Atzori C, Angeli E, Agostoni F, Mainini A, Micheli V, Cargnel A
Abstract excerpt
OBJECTIVES: To verify the clinical value of two different polymerase chain reactions (PCRs) for noninvasive diagnosis and follow-up during Pneumocystis carinii f. sp. hominis pneumonia (PCP) and to analyze the P. carinii f. sp. hominis genotypes involved. METHODS: Internal transcribed spacers (ITSs) nested PCR was applied to 630 samples (bronchoalveolar lavage, sera, peripheral blood mononuclear cells, and...
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