Article
Genetic diversity of Pneumocystis carinii f. sp. hominis based on variations in nucleotide sequences of internal transcribed spacers of rRNA genes.
Journal of clinical microbiology - 1 Apr 2002
Nimri Laila F, Moura Iaci N S, Huang Laurence, del Rio Carlos, Rimland David, Duchin Jeffrey S, Dotson Ellen M, Beard Charles B
Abstract excerpt
A variety of genes have been used to type Pneumocystis carinii. In the present study, nucleotide sequence variations in the ITS1 and ITS2 internal transcribed spacer (ITS) regions of the rRNA genes were used to type Pneumocystis carinii f. sp. hominis DNA obtained from the lungs of 60 human immunodeficiency virus-infected individuals. These regions were amplified by PCR, cloned, and sequenced. Multibase...
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