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Article

Growth Retardation of Inner Cell Mass Cells in Polyspermic Porcine Embryos Produced In Vitro1

1 May 1999

Abstract excerpt

The in vitro viability of polyspermic pig eggs was investigated. Immature oocytes were matured and fertilized in vitro. Approximately 10 h after insemination, the eggs were centrifuged at 12 000 x g for 10 min and individually classified into two (2PN)- and poly-pronuclear (PPN, 3 or 4 pronuclei) eggs. The classified eggs were cultured in vitro or in vivo. Nuclei numbers of inner cell mass (ICM) and trophectoderm...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 10208971
DOI: 10.1095/biolreprod60.5.1110

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