Characterization of bacteriophage lambda Q- mutant for stable and efficient production of recombinant protein in Escherichia coli system.

Abstract excerpt

We previously demonstrated that the lambda system integrated into the host chromosome can overcome the instability encountered in continuous operations of unstable plasmid-based expression vectors. High stability of a cloned gene in a lysogenic state and a high copy number in a lytic state provide cloned-gene stability and overexpression in a two-stage continuous operation. But the expression by the commonly used...

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