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Production of Cloned Calves Following Nuclear Transfer with Cultured Adult Mural Granulosa Cells1

1 Apr 1999

Abstract excerpt

Adult somatic cell nuclear transfer was used to determine the totipotent potential of cultured mural granulosa cells, obtained from a Friesian dairy cow of high genetic merit. Nuclei were exposed to oocyte cytoplasm for prolonged periods by electrically fusing quiescent cultured cells to enucleated metaphase II cytoplasts 4-6 h before activation (fusion before activation [FBA] treatment). Additionally, some...

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PMID: 10084977
DOI: 10.1095/biolreprod60.4.996

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