Article
Isolation, sequencing, and mutagenesis of the gene encoding cytochrome c553i of Paracoccus denitrificans and characterization of the mutant strain.
Journal of bacteriology - 1 Nov 1991
Ras J, Reijnders W N, Van Spanning R J, Harms N, Oltmann L F, Stouthamer A H
Abstract excerpt
The periplasmically located cytochrome c553i of Paracoccus denitrificans was purified from cells grown aerobically on choline as the carbon source. The purified protein was digested with trypsin to obtain several protein fragments. The N-terminal regions of these fragments were sequenced. On the...
Topics
- Amino Acid Sequence
- Base Sequence
- Cytochrome c Group
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Genes
- Genes, Bacterial
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Open Reading Frames
- Paracoccus denitrificans
- Restriction Mapping
- Sequence Alignment