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Article

Isolation, sequencing, and mutagenesis of the gene encoding cytochrome c553i of Paracoccus denitrificans and characterization of the mutant strain.

Journal of bacteriology - 1 Nov 1991

Ras J, Reijnders W N, Van Spanning R J, Harms N, Oltmann L F, Stouthamer A H

Abstract excerpt

The periplasmically located cytochrome c553i of Paracoccus denitrificans was purified from cells grown aerobically on choline as the carbon source. The purified protein was digested with trypsin to obtain several protein fragments. The N-terminal regions of these fragments were sequenced. On the basis of one of these sequences, a mix of 17-mer oligonucleotides was synthesized. By using this mix as a probe, the...

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Identifiers

PMID: 1657873
DOI: 10.1128/jb.173.21.6971-6979.1991
PMCID: PMC209051

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Isolation, sequencing, and mutagenesis of the gene encoding cytochrome c553i of Paracoccus denitrificans and characterization of the mutant strain.PMID 1657873Read ↗

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