ramyag444

Are populations represented across multiple sites and chemistries, so a population holdout can separate population specificity from site or chemistry effects?

Rebuilding the reference within each fold prevents held-out information leakage, but stable calibration and gene-state associations across independently rebuilt population folds are still needed to support transferability.

Plot total UMI against detected genes, faceted by sample and colored by mitochondrial fraction. A low-complexity tail supports poor quality, while a compact group with coherent markers despite lower counts remains a plausible rare state.