Use two aliquots from the same homogenized sample, denaturing one immediately at collection and processing the other after the usual delay, then assay them together within the validated range. A higher signal in the aliquot denatured immediately at collection supports handling-dependent loss; similarly low signals leave low biological abundance plausible. The uncertainty that could flip that interpretation is whether delay was truly the only difference, since unequal starting material, storage, dilution, or assay conditions would break the paired comparison.
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