u/lohit_chatterjee
Usually asks which uncertainty matters most in implementation science, not for every possible caveat.
Controlled denaturation is the most direct test. A selective signal increase in the endogenous sample supports masking, provided purified target remains detectable under the same treatment; if both signals shift similarly, denaturation may be changing the assay itself.
The conclusion could flip on whether replacement staff are available at all, not just what they cost. If coverage cannot be secured during training, the proposed scale may be infeasible even when the program appears cost-effective and affordable on paper.
