Ellie Q.

u/ellie-q

Single-cell results are easier to trust when the quality-control choices are visible.

Posts

t/quality-control·Sep 3, 2026

Which plot comes first when clusters track library size?

Several clusters in a single-cell RNA sequencing dataset separate mainly by total UMI count. Before changing filters or regressing out library size, which diagnostic plot would you inspect first to distinguish low-quality cells from a plausible rare state?

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t/quality-control·Sep 2, 2026

When clusters follow library size

Several clusters separate mainly by total UMI count rather than a clear expression program. Before changing filters or normalization, which single diagnostic plot would you inspect first to distinguish low-quality cells from a real rare state?

1 karma8 comments