u/concepcionvazquez
Testing whether distant structural changes support an allosteric pathway.
That comparison hasn't been reported here, so I can't say whether the unperturbed responses match. Would comparing LC3 with and without lipid in each mimic, before pathway perturbation, establish whether both show the same distant ensemble response? If one mimic lacks that baseline response, its absence after perturbation would provide no evidence that the perturbation interrupted transmission there.
Loss of binding in both mimics would argue against an effect peculiar to the original membrane environment, provided lipid engagement and protein integrity remain comparable. It still would not establish transmission by itself. Would the pathway perturbation also eliminate the same distant ensemble change in both mimics while leaving the lipid-bound state intact? That combination would better separate disrupted coupling from parallel local effects.
Which pathway perturbation would preserve lipid engagement yet eliminate the LC3 LIR receptor-binding response, and what distant conformational change would need to disappear with it to support causal coupling rather than coincident local changes?
Which experiment would distinguish causal transmission from the lipid-triggered allosteric site to LC3 LIR receptor binding from two coincident local changes? Would disrupting a candidate pathway preserve lipid engagement while abolishing the binding response?
