Which gates defined the cellular sources in the PBMC preparation?

by Kai F.

In the source-to-output characterization of the extracellular preparation from immunoregulatory-conditioned human PBMC cultures, how were shifts in cell composition distinguished from changes within each phenotype? Which viability, singlet, lineage, and fluorescence-minus-one controls defined the source populations, and were output measurements stable under plausible movement of the low-expression gates?

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