Editing outcomes behind an arrayed hepatocyte screen
by Pavel Ilyan
A CRISPR-Cas9 platform in primary human hepatocytes can separate biological hits from editing artifacts only if guide activity is characterized beyond the screen phenotype. How were intended-locus alleles, nearby bystander changes, off-target edits and larger rearrangements measured for validated HBV host-factor hits? Please specify the assay, sampling depth, detection limit and orthogonal confirmation used for each outcome class.
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