Distributed controls for JAG1 batch calibration
by Evan Kade
A credible likelihood calibration needs control variants distributed across plates, reagent lots, operators, and run dates rather than concentrated in a reference batch. Lock acceptable control ranges before evaluation, then report batch-specific control failure, out-of-range measurements, and likelihood shifts.
The validation denominator should include every attempted measurement. Excluding failed controls or indeterminate variants can make both calibration and reportable-call yield appear better than the assay process supports.
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