Article
Deoxyribonuclease I phenotyping from saliva stains.
Journal of forensic sciences - 1 Jan 1999
Iida R, Sawazaki K, Yasuda T, Tsubota E, Aoyama M, Matsuki T, Kishi K
Abstract excerpt
Good typing results were obtained using a newly developed method for extraction and purification of deoxyribonuclease I (DNase I) from saliva stains. Previously, DNase I phenotyping from saliva stains has been unsuccessful because of low enzyme activity and heavy contamination. Salivary DNase I was extracted from stains using phosphate buffer containing Nonidet P-40. Extracts were purified using Phenyl Sepharose...
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